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Registros recuperados: 35
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Allozyme variation and sociogenetic structure of Polistes satan Bequaert 1940 colonies (Hymenoptera, Vespidae) Genet. Mol. Biol.
Gaspar,Jane Cristina Wehren; López-Uribe,Margarita María; Del Lama,Marco Antônio.
We collected 40 colonies of the wasp Polistes satan in November 1993 (Population A = 23 colonies) and October 1995 (Population B = 17 colonies) from the town of Delfinopólis in the Brazilian state of Minas Gerais. Pupae from these nests were used to assess allozyme variation and genetic relatedness among nestmates. Of the 24 gene loci sampled six showed allozyme polymorphism (Est-2, Pgm-1, 6Pgd, Hk-1, Idh and Pep-A) and all loci except Idh were at Hardy-Weinberg equilibrium. Populations A and B showed distinct intra-loci heterozygosities, but similar average heterozygosity over all loci. Significant inter-population differentiation (Fst = 0.051; p < 0.01) was detected, probably due to genetic drift driven by bottlenecking that occurred due to the first...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allozymes; Gene variation; Polistes satan; Relatedness; Wasps.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300028
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Allozyme variation among three populations of the armored catfish Hypostomus regani (Ihering, 1905) (Siluriformes, Loricariidae) from the Paraná and Paraguay river basins, Brazil Genet. Mol. Biol.
Zawadzki,Cláudio H.; Renesto,Erasmo; Peres,Maria Dolores; Paiva,Suzana.
Three Brazilian populations of the armored catfish Hypostomus regani (Ihering, 1905) were sampled, one from the Corumbá Reservoir in Goiás state, another from the Itaipu Reservoir in Paraná state and a third from the Manso Reservoir in Mato Grosso state. Allozyme electrophoresis was used to establish the genetic structure of the species, with the analysis of liver, heart and muscles tissues allowing the scoring of 25 loci from 14 enzymatic systems. Although no diagnostic loci were found, some exclusive rare alleles were recorded for the three populations. The genetically most similar populations were those from Corumbá and Itaipu, and the most distant were the populations from Manso and Corumbá. The allozyme data showed three structured populations...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allozymes; Genetic variability; Hypostomus regani; Loricariidae; Paraguay and Paraná Rivers.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400025
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Genetic structure of honeybee populations from southern Brazil and Uruguay Genet. Mol. Biol.
Diniz,Nilza Maria; Soares,Ademilson Espencer Egea; Sheppard,Walter Steve; Del Lama,Marco Antonio.
Apis mellifera scutellata was introduced to Brazil in 1956 and Africanized honeybee populations have now spread from Argentina to the southwestern United States. Temperate climatic restrictions seem to be a natural limit to Africanized honeybee expansion around parallels 35° to 40° SL. We used allozyme loci (Mdh-1 and Hk-1) and mtDNA haplotypes to characterize honeybee populations in southern Brazil and Uruguay and define a possible transition area between Africanized and European bees. Samples of 194 bee colonies were collected from ten localities between 30°-35° SL and 52°-59° WL. The mtDNA restriction patterns of these colonies were obtained through digestion of the mitochondrial genome by Eco RI, or by digestion by Bgl II and Xba I of the cytochrome B...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Africanized honeybees; Transition area; South America; MtDNA; Allozymes; Population genetics.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100008
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Biochemical comparison of two Hypostomus populations (Siluriformes, Loricariidae) from the Atlântico Stream of the upper Paraná River basin, Brazil Genet. Mol. Biol.
Ito,Kennya F.; Renesto,Erasmo; Zawadzki,Cláudio H..
Two syntopic morphotypes of the genus Hypostomus - H. nigromaculatus and H. cf. nigromaculatus (Atlântico Stream, Paraná State) - were compared through the allozyme electrophoresis technique. Twelve enzymatic systems (AAT, ADH, EST, GCDH, G3PDH, GPI, IDH, LDH, MDH, ME, PGM and SOD) were analyzed, attributing the score of 20 loci, with a total of 30 alleles. Six loci were diagnostic (Aat-2, Gcdh-1, Gpi-A, Idh-1, Ldh-A and Mdh-A), indicating the presence of interjacent reproductive isolation. The occurrence of few polymorphic loci acknowledge two morphotypes, with heterozygosity values He = 0.0291 for H. nigromaculatus and He = 0.0346 for H. cf. nigromaculatus. F IS statistics demonstrated fixation of the alleles in the two morphotypes. Genetic identity (I)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allozymes; Hypostomus nigromaculatus; Fish genetics; Genetic distance; Polymorphism.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100008
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Genetic variability in Astyanax altiparanae Garutti &amp; Britski, 2000 (Teleostei, Characidae) from the Upper Paraná River basin, Brazil Genet. Mol. Biol.
Peres,Maria Dolores; Vasconcelos,Marcio dos Santos; Renesto,Erasmo.
Allozyme data was used to assess the genetic diversity Astyanax altiparanae populations from the floodplain of the Upper Paraná River (PR). Specimens were collected in the southern Brazilian state of Paraná from PR in Porto Rico municipality and Ribeirão Ficha (RF) in Ubiratã municipality. The authors used 15% (w/v) corn starch gel electrophoresis to identify 21 putative loci for 13 enzymatic systems: Aspartate aminotransferase, 2.6.1.1 (AAT), Acid phosphatase, 3.1.3.2 (ACP), Esterase, 3.1.1.1 (EST), Glycerol-3-phosphate dehydrogenase, 1.1.1.8 (G3PDH), Glucose-6-phosphate dehydrogenase, 1.1.1.49 (G6PDH), Glucose-6-phosphate isomerase, 5.3.1.9 (GPI), Iditol dehydrogenase, 1.1.1.14 (IDDH), Isocitrate dehydrogenase - NADP+, 1.1.1.42 (IDH), L-Lactate...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allozymes; Astyanax; Fishes; Genetic variability; Paraná River floodplain.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500011
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Genetic and morphometric differences between yellowtail snapper (Ocyurus chrysurus, Lutjanidae) populations of the tropical West Atlantic Genet. Mol. Biol.
Vasconcellos,Anderson V.; Vianna,Paulo; Paiva,Paulo C.; Schama,Renata; Solé-Cava,Antonio.
Populations of Ocyurus chrysurus were compared genetically and morphometrically along the West Atlantic coast to test the null hypothesis of population homogeneity in the area. Brazilian populations were found to be differentiated in shape (canonical variates analysis; F[48,515] = 10.84, p < 0.0001). Analyses of mitochondrial DNA sequences (663 bp of the control region) did not show any differences between Brazilian populations but could detect differences between Brazilian and Caribbean (Belize) populations. The samples from Pernambuco differed significantly from the other Brazilian populations in allozyme frequencies (11 loci; F ST = 0.167; p < 0.05), but this may have resulted from the small number of samples analysed for that population. Sequence...
Tipo: Info:eu-repo/semantics/article Palavras-chave: MtDNA; Allozymes; Morphometry; Fisheries; Phylogeography.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200026
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Allozyme differentiation of two populations of the genus Neoplecostomus Eigenmann &amp; Eigenmann, 1888 (Teleostei, Loricariidae) from the upper Paraná River basin, Brazil Genet. Mol. Biol.
Reusing,Ana Flávia; Renesto,Erasmo; Roxo,Fábio F.; Zawadzki,Cláudio H..
Allozyme electrophoresis was used to examine 12 enzymatic systems in two populations of the genus Neoplecostomus from the Paraná River basin. Samples of Neoplecostomus sp. 1 were collected in Paraitinguinha stream of the Tietê River basin, in the municipality of Salesópolis, São Paulo State, and those of Neoplecostomus sp. 2 from São Domingos stream of the Rio Grande River basin, in the municipality of Muzambinho, Minas Gerais State. The genetic variability of the two populations was estimated by Nei's expected heterozygosity and was considered lower than average for populations of freshwater fish. The proportion of polymorphic loci was low (only 5.26% for the locus Idh). The low frequency of heterozygosity for both populations revealed a high fixation of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allozymes; Genetic variability; Neoplecostominae; Neotropical fishes.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300021
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Population genetic structure of Brazilian shrimp species (Farfantepenaeus sp., F. brasiliensis, F. paulensis and Litopenaeus schmitti: Decapoda: Penaeidae) Genet. Mol. Biol.
Gusmão,Jaqueline; Lazoski,Cristiano; Solé-Cava,Antonio M..
Penaeid shrimps are important resources for worldwide fisheries and aquaculture. In the Southwest Atlantic, Farfantepenaeus brasiliensis, F. paulensis, F. subtilis, Farfantepenaeus sp. and Litopenaeus schmitti are among the most important commercially exploited species. Despite their high commercial value, there is little information available on the different aspects of their biology or genetics and almost no data on their stock structure. We used allozymes to estimate variability levels and population genetic structure of F. brasiliensis, F. paulensis, L. schmitti and the recently detected species Farfantepenaeus sp. along as much as 4,000 km of Brazilian coastline. No population heterogeneity was detected in F. brasiliensis or L. schmitti along the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Farfantepenaeus; Litopenaeus; Allozymes.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100029
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Genetic variability of Hypostomus (Teleostei, Loricariidae) from the Ribeirão Maringá, a stream of the upper Rio Paraná basin, Brazil Genet. Mol. Biol.
Paiva,Suzana de; Renesto,Erasmo; Zawadzki,Cláudio Henrique.
Hypostomus strigaticeps and two morphotypes of Hypostomus were collected from Ribeirão Maringá, a small tributary of the Rio Pirapó, an effluent of the upper Rio Paraná. The three populations were analyzed by allozyme electrophoresis that allowed the scoring of 25 loci from 14 enzyme systems. Heterozygosity values (He) were 0.028 in H. strigaticeps, 0.027 in Hypostomus sp. 1 and zero in Hypostomus sp. 2. Several diagnostic loci and fixed differences were observed for each population at loci Acp-A, Gcdh-A and Mdhp-A. Thus, all populations were genetically distinct, although there were many common alleles. The unbiased genetic identities of Nei (I) were estimated as 0.780 for Hypostomus sp. 1 and H. strigaticeps, 0.357 for H. strigaticeps and Hypostomus sp....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allozymes; Heterozygosity; Hypostomus; Loricariidae; Systematic.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300005
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Allozyme comparison of two populations of Rineloricaria (Siluriformes, Loricariidae) from the Ivaí River, upper Paraná River basin, Brazil Genet. Mol. Biol.
Limeira,Daniel M.; Renesto,Erasmo; Zawadzki,Cláudio H..
Two allopatric morphotypes of the genus Rinelocaria were compared through the allozyme electrophoresis technique: one morphotype, R. pentamaculata, from the Keller River in the middle stretch of the Ivaí River basin and the other, R. aff. pentamaculata, from the São João River in the upper portion of the Ivaí River basin. The morphotype from the São João River was collected upstream from the São João waterfall, which is about 80 m deep. Twelve enzymatic systems (AAT, ADH, EST, GCDH, G3PDH, GPI, IDH, LDH, MDH, ME, PGM and SOD) were analyzed, which allowed to score 22 loci. Only loci Aat-2, Est-3 and Mdh-C showed polymorphism. The two samples differed in allele frequencies at the three polymorphic loci. The average expected heterozygosity for all loci was...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Allozymes; Fish genetics; Genetic distance; Polymorphism; Loricariidae; Pisces.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200034
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Populational genetic structure and sociogenetic structure of cocoon masses of Digelasinus diversipes (Kirby, 1882) (Hymenoptera: Symphyta: Argidae) Genet. Mol. Biol.
Boraschi,Daniele; Del Lama,Marco Antonio.
Gene variation and population genetic structure of the Neotropical sawfly Digelasinus diversipes were measured by allozyme analyses using starch gel electrophoresis. Cocoon masses were collected in Eugenia glazioviana (Myrtaceae) stems, in two areas of the "Estação Ecológica Jataí" (Luiz Antônio, SP, Brazil - 21°25' S, 47°50' W), in 2000 and 2001. The average heterozygosity observed in this species (Hobs = 0.094 ± 0.025) was not significantly different from other Symphyta groups; it was, however, significantly higher than in other Hymenoptera populations. No significant levels of inbreeding were found (F IS = 0.062; chi2 = 29.9; p > 0.05), but the population was subdivided (F ST = 0.070; chi2 = 458.9; p < 0.05), suggesting the absence of a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic variability; Population genetics; Digelasinus diversipes; Neotropical sawfly; Allozymes.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300013
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Genetic structure of bisexual and parthenogenetic populations of Artemia from Italian brackish-hypersaline waters ArchiMer
Nascetti, G; Bondanelli, P; Aldinucci, A; Cimmaruta, R.
Eight populations of Artemia displaying different reproduction modalities (amphigonic vs. parthenogenetic) and ploidies (diploid vs. polyploid) have been collected from Italian salt-works or brackish coastal lagoons, and their genetic structure has been investigated using allozyme electrophoresis. The results obtained on genetic differentiation showed that the Tyrrhenian bisexual samples (A. salina) are genetically homogeneous even between populations located in relatively faraway sites (Sardinia-Latium), having a moderate gene flow assisted by migratory waterfowl. The parthenogenetic samples (A. parthenogenetica) are genetically well differentiated both from the bisexual ones and among them, even when characterised by the same ploidy. Each sample presents...
Tipo: Text Palavras-chave: Artemia; Allozymes; Reproduction amphigonique; Parthénogenèse; Hétérozygotie; Artemia; Allozymes; Amphigony; Parthenogenesis; Heterozygosity.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/00322/43289/43024.pdf
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Variance of reproductive success in the European flat oyster Ostrea edulis assessed by microsatellite-based parentage analyses in a natural population and in hatchery ArchiMer
Lallias, Delphine; Taris, Nicolas; Boudry, Pierre; Bonhomme, Francois; Lapegue, Sylvie.
he European flat oyster (Ostrea edulis L.) is a marine bivalve whose natural geographical distribution ranges along the European Atlantic coast from Norway to Morocco, in addition to the Mediterranean and Black Sea. Previous studies of allozymes, microsat-ellites and mitochondrial differentiation over the whole range concluded that a significant divergence existed between Mediterranean and Atlantic populations, together with an isolation-by-distance pattern. However, the average mitochondrial haplotypic diversity displayed a high among populations variance, reflecting smaller effective population size in some locations. Additionally, a ten-fold quantitative difference was observed in the same study in Fst between the mitochondrial and the nuclear genomes,...
Tipo: Text Palavras-chave: Genotypes; Genetic diversity; Breeding success; Allozymes; Population genetics; Parentage analyses; Natural population; Ostrea edulis; Oyster.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/acte-3334.pdf
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Habitat selection and phenotypic diversity in the anchovy of the Bay of Biscay ArchiMer
Petitgas, Pierre; Grellier, Patrick; Leseur, Floriane; Lenfant, Philippe.
Diversity among individuals in a population is an important feature linking vital rates with behaviour and spatial occupation. We measured the growth increments in the otolith of individual fishes collected on the annual fisheries survey PELGAS from 2001 to 2015. Individuals who grew larger at juvenile stage occupied later in life more off-shore habitats. Further, we analysed the allozymes of 13 different loci from 2001 to 2006. Alleles of the enzyme IDH showed different frequencies in inshore and offshore habitats. The population spatially segregates along a coast to off-shore gradient with individuals showing different early growth and allele frequencies. Results show how individuals in a population segregate spatially in different habitats in relation...
Tipo: Text Palavras-chave: Allozymes; Otoliths; Habitats; Spatial distribution; Anchovy.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00353/46379/46001.pdf
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Sharp genetic break between Atlantic and English Channel populations of the polychaete Pectinaria koreni, along the North coast of France ArchiMer
Jolly, Mt; Jollivet, D; Gentil, F; Thiebaut, E; Viard, F.
This study uses enzymatic and mitochondrial genes to infer the relative importance of historical processes and contemporary hydrodynamic features on the observed patterns of genetic structure in subdivided populations of Pectinaria koreni (Polychaeta: Pectinariidae) along the coasts of Brittany and the English Channel. Nucleotide sequence variation of a 603-bp fragment of the mtDNA cytochrome oxidase subunit I gene revealed a surprisingly deep phylogeographic break of about 16% divergence separating the Brittany and Channel populations, which coincides with a biogeographic boundary along the western coast of Brittany. Deep sequence divergence with fixed haplotype differences and the inversion of allele frequencies at two enzyme loci suggests the occurrence...
Tipo: Text Palavras-chave: Phylogeography; Allozymes; MtDNA; Parapatric boundary; Cryptic species.
Ano: 2005 URL: https://archimer.ifremer.fr/doc/00301/41250/40433.pdf
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Genetic diversity and environmental stress in Italian populations of the cyprinodont fish Aphanius fasciatus ArchiMer
Cimmaruta, R; Scialanca, F; Luccioli, F; Nascetti, G.
Allozyme electrophoresis has been used to analyse the genetic structure of ten populations of the killifish, Aphanius fasciatus, sampled from Italian coastal brackish lagoons or salterns. The goal of the study was to compare the genetic structure of samples obtained from habitats characterised by environmental stress of different kinds, with the aim of gaining a better understanding of the relationships between the genetic variation of natural populations and environmental disturbance, either natural or man-made. Therefore, the sampling scheme has been planned to provide samples from both brackish habitats, characterised by natural stress with seasonal marked fluctuations of chemical and physical parameters, and from coastal lagoons suffering from a high...
Tipo: Text Palavras-chave: Aphanius fasciatus; Hétérozygotie; Allozymes; Eaux saumâtres; Stress du milieu; Aphanius fasciatus; Heterozygosity; Allozymes; Brackish waters; Environmental stress.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/00322/43290/43025.pdf
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Estimation du polymorphisme enzymatique d'une population de Penaeus jap nicus maintenue en élevage depuis quatre générations ArchiMer
Laubier, A; Pasteur, N; Moriyasu, M.
The polymorphism of 37 enzyme-encoding genes was investigated in a Penaeus japonicus population born the same night from a stock maintained artificially four generations. Five loci were found to be polymorphie (Lap-4, Es-3, Es-6, Es-8 and Mpi-2), and genotype frequencies at four of these loci indicate: a) that the shrimps studied come from the same two parents and b) that Mpi-2 and Es-3 genes are autosomal. The rate of polymorphism observed in this Penaeus japonicus population is similar to that described in natural populations of other Peneidae species, despite the probable occurrence of inbreeding.
Tipo: Text Palavras-chave: Penaeus japonicus; Aquaculture; Génétique; Allozym; Penaeus japonicus; Aquaculture; Genetics; Allozymes.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/00113/22443/20130.pdf
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Remodeling of membrane lipids in gills of adult hard clam Mercenaria mercenaria during declining temperature ArchiMer
Parent, G; Pernet, Fabrice; Tremblay, R; Sevigny, J; Ouellette, M.
In a previous study, a major remodeling of lipids, consistent with the theory of homeoviscous adaptation, was observed in the juvenile hard clam Mercenaria mercenaria during a temperature reduction from 24 to -1 degrees C. In addition, the lipid remodeling varied between genetically distinct lines of hard clams. The present study examined whether adult hard clams originating from different locations and a selectively bred variety show differences related to their genetic characteristics in the remodeling of lipids that normally occur during decreasing temperatures. Wild hard clams from 4 locations in Atlantic Canada and the selectively bred M mercenaria var. notata were held at an aquaculture growout site located at the northern distribution limit of the...
Tipo: Text Palavras-chave: Sterol; Phospholipid; Membrane lipid; Intraspecific variation; Food; Fatty acid; Bivalve; Allozymes; Acclimatization.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4743.pdf
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Phylogeny and phylogeography of Atlantic oyster species: evolutionary history, limited genetic connectivity and isolation by distance ArchiMer
Lazoski, C.; Gusmao, J.; Boudry, Pierre; Sole-cava, A. M..
The phylogenetic relationships between naturally occurring Atlantic Crassostrea oyster species were inferred through analyses of mitochondrial (cytochrome oxidase subunit I and 16S) and nuclear (second internal transcribed spacer) sequences. We also scored 15 allozyme loci on 422 oysters to study population structuring of C. rhizophorae and C. brasiliana along 9000 km of the Western Atlantic coastline. Despite morphological similarities, C. virginica was genetically more closely related to C. rhizophorae than to C. brasiliana. In contrast, C. paraibanensis was genetically indistinguishable from C. brasiliana, which is probably a junior synonym of the African C. gasar. Significant genetic differentiation between populations of C. rhizophorae and C. gasar...
Tipo: Text Palavras-chave: Population genetics; Biogeography; Allozymes; Cytochrome c oxidase subunit I; COI; 16S; Internal transcribed spacer 2; ITS-2.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00033/14464/11765.pdf
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Phylogeny and biogeography of midwife toads (Alytes, Discoglossidae): a rebuttal Naturalis
Arntzen, J.W.; García-París, M..
Three competing phylogenetic hypotheses for the genus Alytes (midwife toads) are evaluated. Based on quantitative coding of protein characters the most parsimonious solution shows a sister taxon relationship for Alytes dickhilleni and A. muletensis. The alternatives in which A. obstetricans has its sister group in either A. dickhilleni or A. muletensis lack support. Using calibrations derived from protein evolutionary rates, the vicariant events giving rise to A. obstetricans and the lineage leading to the A. muletensis and A. dickhilleni clade and the subsequent splitting between A. muletensis and A. dickhilleni cannot be placed much earlier than the Miocene-Pliocene boundary. Biogeographical scenarios invoking an earlier time of divergence should be...
Tipo: Article / Letter to the editor Palavras-chave: Allozymes; Alytes; Biogeography; Midwife toads; Phylogenetics; Spain; Taxonomy; Vicariance.
Ano: 1997 URL: http://www.repository.naturalis.nl/record/504096
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